| Title | Counterselection against D mu is mediated through immunoglobulin (Ig)alpha-Igbeta. |
| Publication Type | Journal Article |
| Year of Publication | 1996 |
| Authors | Gong S, Sanchez M, Nussenzweig MC |
| Journal | J Exp Med |
| Volume | 184 |
| Issue | 6 |
| Pagination | 2079-84 |
| Date Published | 1996 Dec 01 |
| ISSN | 0022-1007 |
| Keywords | Animals, B-Lymphocytes, Base Sequence, Crosses, Genetic, DNA Primers, Female, Flow Cytometry, Immunoglobulin alpha-Chains, Immunoglobulin G, Immunoglobulin Heavy Chains, Immunoglobulin Joining Region, Immunoglobulin mu-Chains, Immunoglobulin Variable Region, Mice, Mice, Inbred BALB C, Mice, Transgenic, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Antigen, B-Cell, Signal Transduction |
| Abstract | The pre-B cell receptor is a key checkpoint regulator in developing B cells. Early events that are controlled by the pre-B cell receptor include positive selection for cells express membrane immunoglobulin heavy chains and negative selection against cells expressing truncated immunoglobulins that lack a complete variable region (D mu). Positive selection is known to be mediated by membrane immunoglobulin heavy chains through Ig alpha-Ig beta, whereas the mechanism for counterselection against D mu has not been determined. We have examined the role of the Ig alpha-Ig beta signal transducers in counterselection against D mu using mice that lack Ig beta. We found that D mu expression is not selected against in developing B cells in Ig beta mutant mice. Thus, the molecular mechanism for counterselection against D mu in pre-B cells resembles positive selection in that it requires interaction between mD mu and Ig alpha-Ig beta. |
| Alternate Journal | J. Exp. Med. |
| PubMed ID | 8976164 |
| PubMed Central ID | PMC2196397 |