Counterselection against D mu is mediated through immunoglobulin (Ig)alpha-Igbeta.

TitleCounterselection against D mu is mediated through immunoglobulin (Ig)alpha-Igbeta.
Publication TypeJournal Article
Year of Publication1996
AuthorsGong S, Sanchez M, Nussenzweig MC
JournalJ Exp Med
Date Published1996 Dec 01
KeywordsAnimals, B-Lymphocytes, Base Sequence, Crosses, Genetic, DNA Primers, Female, Flow Cytometry, Immunoglobulin alpha-Chains, Immunoglobulin G, Immunoglobulin Heavy Chains, Immunoglobulin Joining Region, Immunoglobulin mu-Chains, Immunoglobulin Variable Region, Mice, Mice, Inbred BALB C, Mice, Transgenic, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Antigen, B-Cell, Signal Transduction

The pre-B cell receptor is a key checkpoint regulator in developing B cells. Early events that are controlled by the pre-B cell receptor include positive selection for cells express membrane immunoglobulin heavy chains and negative selection against cells expressing truncated immunoglobulins that lack a complete variable region (D mu). Positive selection is known to be mediated by membrane immunoglobulin heavy chains through Ig alpha-Ig beta, whereas the mechanism for counterselection against D mu has not been determined. We have examined the role of the Ig alpha-Ig beta signal transducers in counterselection against D mu using mice that lack Ig beta. We found that D mu expression is not selected against in developing B cells in Ig beta mutant mice. Thus, the molecular mechanism for counterselection against D mu in pre-B cells resembles positive selection in that it requires interaction between mD mu and Ig alpha-Ig beta.

Alternate JournalJ. Exp. Med.
PubMed ID8976164
PubMed Central IDPMC2196397