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Examination of Bacterial Artificial Chromosome (BAC) DNA Quality and Quantity by Pulsed-Field Gel Electrophoresis.

TitleExamination of Bacterial Artificial Chromosome (BAC) DNA Quality and Quantity by Pulsed-Field Gel Electrophoresis.
Publication TypeJournal Article
Year of Publication2019
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Volume2019
Issue11
Date Published2019 11 01
ISSN1559-6095
KeywordsChromosomes, Artificial, Bacterial, DNA, Electrophoresis, Gel, Pulsed-Field
Abstract

Pulsed-field gel electrophoresis (PFGE) is the preferred method to measure the size of an insert in a high-capacity vector. After digestion with a restriction enzyme (e.g., PI-SceI or NotI), the DNA fragments are separated by PFGE through a 1% agarose gel.

DOI10.1101/pdb.prot098020
Alternate JournalCold Spring Harb Protoc
PubMed ID31676579