| Title | Examination of Bacterial Artificial Chromosome (BAC) DNA Quality and Quantity by Pulsed-Field Gel Electrophoresis. |
| Publication Type | Journal Article |
| Year of Publication | 2019 |
| Authors | Heintz N, Gong S |
| Journal | Cold Spring Harb Protoc |
| Volume | 2019 |
| Issue | 11 |
| Date Published | 2019 11 01 |
| ISSN | 1559-6095 |
| Keywords | Chromosomes, Artificial, Bacterial, DNA, Electrophoresis, Gel, Pulsed-Field |
| Abstract | Pulsed-field gel electrophoresis (PFGE) is the preferred method to measure the size of an insert in a high-capacity vector. After digestion with a restriction enzyme (e.g., PI-SceI or NotI), the DNA fragments are separated by PFGE through a 1% agarose gel. |
| DOI | 10.1101/pdb.prot098020 |
| Alternate Journal | Cold Spring Harb Protoc |
| PubMed ID | 31676579 |