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Growth of and Preparation of DNA.

TitleGrowth of and Preparation of DNA.
Publication TypeJournal Article
Year of Publication2020
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Volume2020
Issue10
Date Published2020 10 01
ISSN1559-6095
Abstract

This protocol describes methods for isolation of total DNA from a strain of carrying a recombinant yeast artificial chromosome (YAC). This method is appropriate for preparing DNA that will be subjected to regular agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, polymerase chain reaction (PCR), or other methods that do not require intact high-molecular-weight DNA. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. Drop dialysis should be used to exchange buffers. The expected yield from a 10-mL culture is 2-4 µg of yeast DNA.

DOI10.1101/pdb.prot098145
Alternate JournalCold Spring Harb Protoc
PubMed ID33004551