| Title | Large-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA. |
| Publication Type | Journal Article |
| Year of Publication | 2019 |
| Authors | Heintz N, Gong S |
| Journal | Cold Spring Harb Protoc |
| Volume | 2019 |
| Issue | 11 |
| Date Published | 2019 11 01 |
| ISSN | 1559-6095 |
| Keywords | Chromosomes, Artificial, Bacterial, Deoxyribonucleases, Type II Site-Specific, DNA, Endonucleases, Genetic Techniques, Saccharomyces cerevisiae Proteins |
| Abstract | A critical step for creating bacterial artificial chromosome (BAC) transgenic organisms is the production of high-quality BAC DNA. The method described here provides a source of DNA for further manipulation and modification, but can also be used for preparing modified BAC DNA, resulting from the one-step or two-step strategy, for use in other applications such as BAC transgenesis. An analysis of precautions to take when working with large DNA molecules is also provided. |
| DOI | 10.1101/pdb.prot098012 |
| Alternate Journal | Cold Spring Harb Protoc |
| PubMed ID | 31676578 |