For COVID-19 vaccine updates, please review our information guide. For patient eligibility and scheduling availability, please visit

Large-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA.

TitleLarge-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA.
Publication TypeJournal Article
Year of Publication2019
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Date Published2019 11 01
KeywordsChromosomes, Artificial, Bacterial, Deoxyribonucleases, Type II Site-Specific, DNA, Endonucleases, Genetic Techniques, Saccharomyces cerevisiae Proteins

A critical step for creating bacterial artificial chromosome (BAC) transgenic organisms is the production of high-quality BAC DNA. The method described here provides a source of DNA for further manipulation and modification, but can also be used for preparing modified BAC DNA, resulting from the one-step or two-step strategy, for use in other applications such as BAC transgenesis. An analysis of precautions to take when working with large DNA molecules is also provided.

Alternate JournalCold Spring Harb Protoc
PubMed ID31676578