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Large-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA.

TitleLarge-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA.
Publication TypeJournal Article
Year of Publication2019
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Volume2019
Issue11
Date Published2019 11 01
ISSN1559-6095
KeywordsChromosomes, Artificial, Bacterial, Deoxyribonucleases, Type II Site-Specific, DNA, Endonucleases, Genetic Techniques, Saccharomyces cerevisiae Proteins
Abstract

A critical step for creating bacterial artificial chromosome (BAC) transgenic organisms is the production of high-quality BAC DNA. The method described here provides a source of DNA for further manipulation and modification, but can also be used for preparing modified BAC DNA, resulting from the one-step or two-step strategy, for use in other applications such as BAC transgenesis. An analysis of precautions to take when working with large DNA molecules is also provided.

DOI10.1101/pdb.prot098012
Alternate JournalCold Spring Harb Protoc
PubMed ID31676578