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Manipulation of gene expression in the central nervous system with lentiviral vectors.

TitleManipulation of gene expression in the central nervous system with lentiviral vectors.
Publication TypeJournal Article
Year of Publication2011
AuthorsSun B, Gan L
JournalMethods Mol Biol
Volume670
Pagination155-68
Date Published2011
ISSN1940-6029
KeywordsAnimals, Cathepsin B, Cell Line, Cells, Cultured, Central Nervous System, Genetic Vectors, Humans, Lentivirus, Mice, Transfection
Abstract

Viral vector-mediated gene transfer is widely used to manipulate gene expression (overexpression or knock down) in cultures and in different tissues of animals. Vectors based on lentiviruses have particularly useful features. Lentiviral vectors mediate gene transfer into any neuronal cell types and induce sustained expression without significant immune responses after delivery into the nervous system. Lentivirus-mediated expression of therapeutic genes has led to long-term treatment of animal models of neurological disorders, such as spinal injury, Parkinson's disease, Huntington's disease, and Alzheimer's disease. Here, we describe the preparation and purification of lentiviral vectors and methods of lentiviral infection in primary neural cultures and in brain regions of interest by stereotaxic injection.

DOI10.1007/978-1-60761-744-0_12
Alternate JournalMethods Mol. Biol.
PubMed ID20967590
Grant ListAG024447 / AG / NIA NIH HHS / United States