A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.

TitleA monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.
Publication TypeJournal Article
Year of Publication2000
AuthorsGuo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM
JournalHum Immunol
Date Published2000 Aug
KeywordsAnimals, Antibodies, Monoclonal, Antigens, CD, Cell Line, Transformed, Dendritic Cells, Endocytosis, Flow Cytometry, Gene Expression, Humans, Lectins, C-Type, Lipopolysaccharides, Lymphoid Tissue, Membrane Glycoproteins, Mice, Mice, Knockout, Minor Histocompatibility Antigens, Monocytes, Receptors, Cell Surface

DEC-205 is a multilectin receptor for adsorptive endocytosis, expressed in mouse dendritic cells (DC) and some epithelia. DEC-205 is homologous to the macrophage mannose receptor (MMR). A cDNA for murine DEC-205 was used to identify 3 overlapping human DEC-205 clones from a lymphocyte library. The human homologue is a transmembrane protein of 1722 aminoacids with 10 externally disposed C-type lectin domains having 77% identity to the mouse counterpart. The NH(2) terminal cysteine-rich and fibronectin type II domains were expressed and used to immunize mice. A hybridoma, MG38, which specifically recognized the immunogen was obtained from a DEC-205 knockout mouse. The antibody precipitated a 205 kD protein from metabolically labeled, monocyte-derived DCs. MG38 labeled mature monocyte-derived DCs but showed weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identified DEC-205 on thymic cortical epithelium and DCs in the thymic medulla and tonsillar T cell areas. In contrast, an anti-MMR antibody stained DEC-205 negative, macrophages in the thymus cortex, the trabeculae of the thymus and tonsil, as well as efferent lymphatics in the tonsil. Therefore, the MG38 anti-DEC-205 antibody is useful for identifying DCs and reveals clear differences in sites where MMR and DEC-205 are expressed in lymphoid tissues.

Alternate JournalHum. Immunol.
PubMed ID10980384
Grant ListAI 39672 / AI / NIAID NIH HHS / United States