| Title | Neuregulin-1 suppresses muscarinic receptor expression and acetylcholine-activated muscarinic K+ channels in cardiac myocytes. |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Ford BD, Liu Y, Mann MAnne, Krauss R, Phillips K, Gan L, Fischbach GD |
| Journal | Biochem Biophys Res Commun |
| Volume | 308 |
| Issue | 1 |
| Pagination | 23-8 |
| Date Published | 2003 Aug 15 |
| ISSN | 0006-291X |
| Keywords | Acetylcholine, Animals, Cells, Cultured, Chick Embryo, Myocardium, Neuregulin-1, Potassium Channels, Receptor, Muscarinic M4, Receptors, Muscarinic, Reverse Transcriptase Polymerase Chain Reaction |
| Abstract | The neuregulin-1 family of growth factors regulates nicotinic acetylcholine receptor synthesis in skeletal muscle, but its role in cardiac myogenesis remains unclear. Here, we investigate the involvement of neuregulins in the development of cardiac cholinergic responsiveness. Treatment of chick cardiac myocytes with neuregulin-1 inhibited mRNA expression of the M4 muscarinic receptor, but not the M2 receptor. In addition, mRNA levels of GIRK1 were reduced in myocytes by treatment with neuregulin-1. Activation of cholinergic receptors in cultured chick atrial myocytes by carbachol produced an outward potassium current (I(K(ACh))), which was attenuated by 24-48-h pre-treatment with neuregulin-1. These data suggest that neuregulins can regulate cardiac parasympathetic tone and may be involved in the pathogenesis of cardiac arrhythmias and heart failure. |
| Alternate Journal | Biochem. Biophys. Res. Commun. |
| PubMed ID | 12890474 |
| Grant List | NS18458 / NS / NINDS NIH HHS / United States |