| Title | One-Step Bacterial Artificial Chromosome (BAC) Modification: Transformation of the BAC Host with the RecA Vector. |
| Publication Type | Journal Article |
| Year of Publication | 2020 |
| Authors | Heintz N, Gong S |
| Journal | Cold Spring Harb Protoc |
| Volume | 2020 |
| Issue | 7 |
| Pagination | 098129 |
| Date Published | 2020 07 01 |
| ISSN | 1559-6095 |
| Abstract | This protocol outlines the steps for introducing the RecA plasmid into bacterial artificial chromosome (BAC) host cells, and their preparation for subsequent transformation with the reporter plasmid for one-step BAC modification. BAC host cells are rendered chemically competent and transformed with the RecA plasmid, and transformants are selected for tetracycline resistance to ensure the presence of the RecA marker. |
| DOI | 10.1101/pdb.prot098129 |
| Alternate Journal | Cold Spring Harb Protoc |
| PubMed ID | 32611779 |