| Title | Small-Scale Isolation of Bacterial Artificial Chromosome (BAC) DNA and Verification by Polymerase Chain Reaction (PCR). |
| Publication Type | Journal Article |
| Year of Publication | 2019 |
| Authors | Heintz N, Gong S |
| Journal | Cold Spring Harb Protoc |
| Volume | 2019 |
| Issue | 11 |
| Date Published | 2019 11 01 |
| ISSN | 1559-6095 |
| Keywords | Chromosomes, Artificial, Bacterial, DNA, Polymerase Chain Reaction, Reproducibility of Results |
| Abstract | In this protocol, small amounts of selected bacterial artificial chromosome (BAC) DNAs are prepared from 5-mL cultures of the host transformed with the BAC clone. DNA is isolated by an adaptation of the alkaline lysis method. The yield is 0.1-0.4 µg, and the BAC DNA is suitable for analysis by polymerase chain reaction (PCR). Considerations for clone selection are also addressed. |
| DOI | 10.1101/pdb.prot098004 |
| Alternate Journal | Cold Spring Harb Protoc |
| PubMed ID | 31676577 |