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Small-Scale Preparations of Yeast DNA.

TitleSmall-Scale Preparations of Yeast DNA.
Publication TypeJournal Article
Year of Publication2020
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Volume2020
Issue10
Date Published2020 10 01
ISSN1559-6095
Abstract

In this protocol, yeast DNA is prepared by digestion of the cell wall and lysis of the resulting spheroplasts with SDS. This method reproducibly yields several micrograms of yeast DNA that can be efficiently cleaved by restriction enzymes and used as a template in polymerase chain reaction (PCR). Note that yeast colonies can also be used directly in PCR, without purifying yeast DNA.

DOI10.1101/pdb.prot098152
Alternate JournalCold Spring Harb Protoc
PubMed ID32763978