Synaptic activity regulates interstitial fluid amyloid-beta levels in vivo.

TitleSynaptic activity regulates interstitial fluid amyloid-beta levels in vivo.
Publication TypeJournal Article
Year of Publication2005
AuthorsCirrito JR, Yamada KA, Finn MBeth, Sloviter RS, Bales KR, May PC, Schoepp DD, Paul SM, Mennerick S, Holtzman DM
Date Published2005 Dec 22
KeywordsAction Potentials, Alzheimer Disease, Amyloid beta-Peptides, Animals, Brain, Exocytosis, Extracellular Fluid, Female, Hippocampus, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Microdialysis, Nerve Degeneration, Organ Culture Techniques, Patch-Clamp Techniques, Perforant Pathway, Plaque, Amyloid, Presynaptic Terminals, Synaptic Transmission, Synaptic Vesicles

Aggregation of the amyloid-beta (Abeta) peptide in the extracellular space of the brain is central to Alzheimer's disease pathogenesis. Abeta aggregation is concentration dependent and brain region specific. Utilizing in vivo microdialysis concurrently with field potential recordings, we demonstrate that Abeta levels in the brain interstitial fluid are dynamically and directly influenced by synaptic activity on a timescale of minutes to hours. Using an acute brain slice model, we show that the rapid effects of synaptic activity on Abeta levels are primarily related to synaptic vesicle exocytosis. These results suggest that synaptic activity may modulate a neurodegenerative disease process, in this case by influencing Abeta metabolism and ultimately region-specific Abeta deposition. The findings also have important implications for treatment development.

Alternate JournalNeuron
PubMed ID16364896
Grant ListAG11355 / AG / NIA NIH HHS / United States
AG13956 / AG / NIA NIH HHS / United States
DA70261 / DA / NIDA NIH HHS / United States