| Title | Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector. |
| Publication Type | Journal Article |
| Year of Publication | 2020 |
| Authors | Heintz N, Gong S |
| Journal | Cold Spring Harb Protoc |
| Volume | 2020 |
| Issue | 4 |
| Pagination | 098079 |
| Date Published | 2020 04 01 |
| ISSN | 1559-6095 |
| Abstract | Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC. |
| DOI | 10.1101/pdb.prot098079 |
| Alternate Journal | Cold Spring Harb Protoc |
| PubMed ID | 32238593 |