|Title||Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Preparation and Verification of the Recombinant Shuttle Vector.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Heintz N, Gong S|
|Journal||Cold Spring Harb Protoc|
|Date Published||2020 04 01|
Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.
|Alternate Journal||Cold Spring Harb Protoc|