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Working with Bacterial Artificial Chromosomes (BACs) and Other High-Capacity Vectors.

TitleWorking with Bacterial Artificial Chromosomes (BACs) and Other High-Capacity Vectors.
Publication TypeJournal Article
Year of Publication2020
AuthorsHeintz N, Gong S
JournalCold Spring Harb Protoc
Volume2020
Issue10
Date Published2020 10 01
ISSN1559-6095
Abstract

Genetic targeting of specific cell types is fundamentally important for modern molecular-genetic studies. The development of simple methods to engineer high-capacity vectors-in particular, bacterial artificial chromosomes (BACs)-for the preparation of transgenic lines that accurately express a gene of interest has resulted in commonplace usage of transgenic techniques in a wide variety of experimental systems. Here we provide a brief description of each of the four major types of large-capacity vectors, with a focus on the use of BAC vectors.

DOI10.1101/pdb.top097998
Alternate JournalCold Spring Harb Protoc
PubMed ID33004554